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Conversion of an AFLP fragment into one dCAPS marker linked to powdery mildew resistance in melon

Fernando Juan Yuste-Lisbona, Carmen Capel, Juan Capel, Rafael Lozano, María Luisa Gómez-Guillamón, Ana Isabel López-Sesé

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Abstract

Resistance to powdery mildew races 1, 2 and 5 has been evaluated in F2/F3\nfamilies from a cross between the resistant line ‘TGR-1551’ and the susceptible\nSpanish cultivar ‘Bola de oro’. Resistance is controlled by two independent genes,\none dominant and one recessive, that both confer resistance to races 1, 2 and 5 of\nPodosphaera xanthii. Bulked segregant analysis (BSA) and amplified fragment\nlength polymorphism (AFLP) technique have been used for the identification of\nmarkers linked to powdery mildew resistance. The AFLP marker E38M82 has been\nconverted into a derived cleaved amplified polymorphic sequences (dCAPS) marker.\nThe marker developed was codominant and the estimated distance with the dominant\ngene for powdery mildew resistance was 5.7 cM.

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What this paper is about

Resistance to powdery mildew races 1, 2 and 5 has been evaluated in F2/F3\nfamilies from a cross between the resistant line ‘TGR-1551’ and the susceptible\nSpanish cultivar ‘Bola de oro’. Resistance is controlled by two independent genes,\none dominant and one recessive, that both confer resistance to races 1, 2 and 5 of\nPodosphaera xanthii. Bulked segregant analysis (BSA) and amplified fragment\nlength polymorphism (AFLP) technique have been used for the identification of\nmarkers linked to powdery mildew resistance. The AFLP marker E38M82 has been\nconverted into a derived cleaved amplified polymorphic sequences (dCAPS) marker.\nThe marker developed was codominant and the estimated distance with the dominant\ngene for powdery mildew resistance was 5.7 cM.

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Available abstract

Resistance to powdery mildew races 1, 2 and 5 has been evaluated in F2/F3\nfamilies from a cross between the resistant line ‘TGR-1551’ and the susceptible\nSpanish cultivar ‘Bola de oro’. Resistance is controlled by two independent genes,\none dominant and one recessive, that both confer resistance to races 1, 2 and 5 of\nPodosphaera xanthii. Bulked segregant analysis (BSA) and amplified fragment\nlength polymorphism (AFLP) technique have been used for the identification of\nmarkers linked to powdery mildew resistance. The AFLP marker E38M82 has been\nconverted into a derived cleaved amplified polymorphic sequences (dCAPS) marker.\nThe marker developed was codominant and the estimated distance with the dominant\ngene for powdery mildew resistance was 5.7 cM.

Key concepts: Powdery mildew, Amplified fragment length polymorphism, Bulked segregant analysis, Biology, Genetics, Cleaved amplified polymorphic sequence, Molecular marker, Cultivar

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Conversion of an AFLP fragment into one dCAPS marker linked to powdery mildew resistance in melon — Research Paper | ScholarLens