Preparation and Identification of the Complete antigen with Enrofloxacin and Production of Polyclonal Antibodies Against Enrofloxacin

Ovalbumin( OVA) and bovine serum albumin( BSA) were used as protein carriers to synthesize small molecule drugs EFLX complete antigens by the active ester method.The conjugates formed in this way were EFLX-BSA and EFLX-OVA.The serum titer was detected by means of EFLX-BSA immunogen and EFLX-OVA coating antigen by ELISA,the specificity and detection limit( LD) were detected by Ci-ELISA. The results showed that the immunogen and coating antigen were successfully synthesized.The serum titer detection result of the first group was 25 600,those of the second and third groups were more than 51 200,that of the second was the highest of all.The result of Ci-ELISA indicated that this method was sensitive and had a linear detection range from 7.39 ng /mL to 403.43 ng /mL( R2= 0.993 9),with IC50and LOD values of 54.60 ng /mL and1.17 ng /mL.This experiment obtained high antibody titer and the specificity was strong,which provides good antibody materials for the development of colloidal gold test and ELISA kit.