1996•Journal of Clinical MicrobiologyOpen access

Identification of a 25-kilodalton protein of Mycobacterium bovis BCG to distinguish BCG strains from Mycobacterium tuberculosis

Deep Kumar, Brahm S. Srivastava, Nidhi Singh, Rakesh K. Srivastava

Abstract

Mycobacterium bovis BCG vaccine strains were compared with Mycobacterium tuberculosis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A 25-kDa protein observed in the BCG strains was absent in M. tuberculosis. Rabbit antibodies specific to the 25-kDa protein uniquely identified this protein in BCG strains but not in M. tuberculosis. It is suggested that the 25-kDa protein and polyclonal antibodies directed against this antigen can be exploited to distinguish BCG strains from M. tuberculosis.

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Key concepts: Mycobacterium bovis, Mycobacterium tuberculosis, Polyclonal antibodies, Tuberculosis, Microbiology, Biology, Antigen, Antibody