1973•Agricultural and Biological ChemistryOpen access

Purification of Mucor Lipases and Their Properties

Kozo NAGAOKA, Yujiro Yamada

Abstract

Olive oil hydrolyzing fraction (F-3) of Mucor lipase was separated into two fractions, F-3A and F-3B, by means of CM-Sephadex column chromatography. Both fractions were homogeneous and F-3A was crystallized. The pH optimum for olive oil hydrolysis of F-3A was at 9.0 and that of F-3B was at 8.0. Chain specificities of the both enzymes were different with each other. Sedimentation coefficient (S20, w) of F-3A was 2.8 S and that of F-3B was 3.1 S. Molecular weights calculated from sedimentation equilibrium data were 25,400 for F-3A and 29,000 for F-3B.

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Key concepts: Chemistry, Mucor, Chromatography, Lipase, Hydrolysis, Fraction (chemistry), Sephadex, Homogeneous