Study on Enzymatic Hydrolysis into Low Molecular Weight Polypeptides with Microwave Heating

Using amino nitrogen content ( AN for short ) as standards, the conditions of low molecular weight polypeptides with three kinds of enzymes hydrolysis as well as the sequence and way of adding enzymes with microwave heating was found in this paper, which was examined by fluorescence analysis method, seperated by Sephadex G-50. The result shows that three kinds of enzymes hydrolysis were prefer to one enzyme obviously, amino nitrogen was higher for 2813.4 mg/L, the relative molecular mass of soybean polypeptides was mostly below 1000 D. Soybean polypeptide was well tested and seperated by Sephadex G-50 and fluorescence analysis method.