Studies on Mucor Lipases

Lipases produced by Mucor lipolyticus Aac-0102 were separated into three different fractions (F-1, F-2 and F-3) by CM-Sephadex column chromatography.Molecular weights of them were estimated to be higher in the order of F-1, F-2, and F-3 by gel filtration on a Sephadex G-100 column.F-1 and F-2 could hydrolyze water soluble substrate such as Tweens.F-3 showed a strong hydrolytic activity toward triglycerides but the activity toward Tweens was almost negligible.Sodium lauryl sulfate inhibited the olive oil hydrolyzing activity of F-3.However, Tween hydrolyzing activity of F-2 was not affected with it.These results suggested that they are different with each other with respect to their molecular weights, substrate specificities and sensitivities to some inhibitors.