Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis

Yeast artificial chromosomes (YACs; 1 ) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects ( 1 – 6 ). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100–1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.