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2002•Journal of Clinical MicrobiologyOpen access

Technical Improvement To Prevent DNA Degradation of Enteric Pathogens in Pulsed-Field Gel Electrophoresis

Joanna Koort, Susanna Lukinmaa, Marjatta Rantala, Erja Unkila, Anja Siitonen

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Abstract

This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.

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What this paper is about

This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.

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Available abstract

This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.

Key concepts: Pulsed-field gel electrophoresis, Salmonella enterica, Serotype, Gel electrophoresis, Biology, Microbiology, Salmonella, Electrophoresis

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