Research on Antibacterial Activity of Crude Extract from Mangifera Indica Leaves

The 95% ethanol extracts of mangifera indica leaf was sequentially extracted with petroleum ether, ethyl acetate, n-butyl alcohol and water. Antibacterial activity and minimal inhibitory concentration (MIC) of the four different extraction against Staphylococlus aureus, Escherichia coli, Bacillus subitlis and Saccaromyces cerevisiae were stuied with filter paper method in this paper. Each of the four extracts showed different antibacterial activity: The water extract is the strongest one; l-butanol extract takes second place; Ethyl acetate and petroleum ether extract is the third. MIC of aqueous extract with the strongest inhibitory effect on Staphylococlus aureus, Escherichia coli and Saccaromyces cerevisiae is 0.0125 g/mL, 0.0125 g/mL, and 0.025 g/mL. Moreover, the aqueous extract showed powerful antibacterial activity against all of four bacteria.