AN ATTEMPT TO INHIBIT THE MULTIPLICATION OF TOBACCO MOSAIC VIRUS IN TISSUE CULTURE BY ITS ANTISERUM
Kazimierz Miczyński
Abstract
Kazimierz Miczyński
Abstract
Extracts from tobacco tissue cultures infected with tobacco mosaic virus grown in medium containing antiserum to the virus had only one‐half to one‐sixteenth as much virus as extracts from tissues grown in medium without the antiserum. When tissues grown with antiserum were thoroughly washed before they were extracted, the extracts contained as much virus as extracts of tissues grown without antiserum. The antiserum did not affect virus multiplication, but antibodies in the tissues may have precipitated virus either in the cells or when the tissues were macerated.
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Extracts from tobacco tissue cultures infected with tobacco mosaic virus grown in medium containing antiserum to the virus had only one‐half to one‐sixteenth as much virus as extracts from tissues grown in medium without the antiserum. When tissues grown with antiserum were thoroughly washed before they were extracted, the extracts contained as much virus as extracts of tissues grown without antiserum. The antiserum did not affect virus multiplication, but antibodies in the tissues may have precipitated virus either in the cells or when the tissues were macerated.
Key concepts: Antiserum, Biology, Virus, Tissue culture, Virology, Tobacco mosaic virus, Antibody, In vitro