2001•Journal of Experimental BotanyRequires access

Leaf senescence in rice plants: cloning and characterization of senescence up‐regulated genes

Ruey‐Hua Lee, Chung‐Hua Wang, Lin‐Tzu Huang, Shu-Chen Grace Chen

Abstract

To identify senescence-associated genes (SAGs) in rice leaves, senescence was induced by transferring rice seedlings into darkness. Senescence up-regulated cDNAs were obtained by PCR-based subtractive hybridization. Among 14 SAG clones characterized, 11 were found to be associated with both dark-induced and natural leaf senescence. Three clones were associated only with dark-induced leaf senescence. The possible physiological roles of these SAGs during rice leaf senescence are discussed.

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Key concepts: Senescence, Biology, Suppression subtractive hybridization, Gene, Cloning (programming), Botany, Cell biology, Genetics