The Principle of Suppression Subtractive Hybridization Technique and its Application in Plant Gene Isolation

Suppression subtractive hybridization (SSH) is a technique for the study of gene expression at transcriptional level, which is based on the suppression PCR and subtractive hybridization. SSH is widely used to study on organism growth and development, senescence and death, as well as the effects of biotic and abiotic stresses on organism due to its high efficiency, stability and reliability. The methodology of SSH and its improvement, application on plant gene isolation are reviewed. Meanwhile, SSH is compared to other different techniques at transcriptional level.;