The effect of microRNA-487b on proliferation and apoptosis of glioma

Objective To investigate the effect of microRNA(miR)-487b on the proliferation and apoptosis of glioma cells by observing the changes of cell proliferation, cell cycle and apoptosis. Methods The expression of miR-487b in 6 samples of glioma tissues and 6 samples of non-tumor control brain tissues was detected by using real-time PCR (qRT-PCR). After artificially synthesized miR-487b mimics was transiently transfected into LN229 glioma cells, the expression of miRNA-487b was tested by qRT-PCR. MTT assay was applied to detect the cell proliferation. The cell cycle and apoptosis were measured by flow cytometry. Results MiR-487b was down-regulated in glioma samples(2.67±0.90)×10-2 compared with non-tumor samples(1.23±0.22). The transient transfection of miR-487b mimics into LN229 glioma cells significantly increased the expression of miR-487b (compared with normal control, P<0.05). The result of MTT assay showed that miR-487b might inhibit LN229 cells proliferation significantly (compared to normal control P<0.05). The cell cycle analysis detected by flow cytometry assay showed that miR-487b raised the cell proportion in G0/G1 phase (compared to normal control, P<0.05). The apoptosis rate detected by flow cytometry assay showed that miR-487b might promote the cell apoptosis (compared with normal control, P<0.05). Conclusion MiR-487b might decrease glioma cells proliferation, block cell cycle, and promote cell apoptosis, which indicated that miR-487b was a new target for the diagnosis and treatment of glioma. Key words: MicroRNA-487b; Glioma; Proliferation; Apoptosis