Overexpression of miR-195 promotes apoptosis in human glioma T98G cells

Objective To investigate the influence of overexpression of miR-195 on cell proliferation in glioma T98G cells and explore the function of miR-195 in glioma.Methods T98G cells with lower expression of miR-195 were transfected with synthesized miR-195 mimics,then MTT assay,flow cytometry and TUNEL assay were performed to analyse the alterations of cellular phenotype,Western blot was performed to examine the expression level of BCL-2.Results Mature miR-195 increased significantly after miR-195 mimics transfection(P0.05).Overexpression of miR-195 induced significant cell proliferation suppression,and up-regulated miR-195 promoted apoptosis and reduced the expression of BCL-2 protein in T98G cells.Conclusions Overexpression of miR-195 significantly promotes apoptosis of T98G cells,suggesting that miR-195 may serve as a potential therapeutic agent for glioma.