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2004•Chinese Journal of Agricultural BiotechnologyRequires access

AFLP markers for genomic DNA fingerprinting in pigs

Jun Ren, Huang Lusheng, Gary Evens, Ai Huashui, Jun Gao, Kefei Chen, Ding Neng-Shui

Open publisher page 3 citations

Abstract

AFLP provides an effective, rapid and economical tool for detecting a large number of polymorphic genetic markers that are highly reliable and reproducible, and are able to be genotyped automatically. The AFLP technique has been used extensively to detect genetic polymorphisms, evaluate and characterize breed resources, construct genetic maps and identify genes. In this paper, we describe the optimization of the AFLP technique for porcine genomic DNA fingerprinting, including the enzyme digestion, adapter ligation, preamplification, selective amplification, denatured PAGE, silver staining and multicolour fluorescent detection. Twenty-eight polymorphic markers were detected in the pooled genomic DNA of 44 pig breeds (populations) by E32/T32 primer combinations.

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What this paper is about

AFLP provides an effective, rapid and economical tool for detecting a large number of polymorphic genetic markers that are highly reliable and reproducible, and are able to be genotyped automatically. The AFLP technique has been used extensively to detect genetic polymorphisms, evaluate and characterize breed resources, construct genetic maps and identify genes. In this paper, we describe the optimization of the AFLP technique for porcine genomic DNA fingerprinting, including the enzyme digestion, adapter ligation, preamplification, selective amplification, denatured PAGE, silver staining and multicolour fluorescent detection. Twenty-eight polymorphic markers were detected in the pooled genomic DNA of 44 pig breeds (populations) by E32/T32 primer combinations.

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Available abstract

AFLP provides an effective, rapid and economical tool for detecting a large number of polymorphic genetic markers that are highly reliable and reproducible, and are able to be genotyped automatically. The AFLP technique has been used extensively to detect genetic polymorphisms, evaluate and characterize breed resources, construct genetic maps and identify genes. In this paper, we describe the optimization of the AFLP technique for porcine genomic DNA fingerprinting, including the enzyme digestion, adapter ligation, preamplification, selective amplification, denatured PAGE, silver staining and multicolour fluorescent detection. Twenty-eight polymorphic markers were detected in the pooled genomic DNA of 44 pig breeds (populations) by E32/T32 primer combinations.

Key concepts: Amplified fragment length polymorphism, Biology, genomic DNA, DNA profiling, Genetic marker, Primer (cosmetics), Genetics, DNA

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