2007•Unpublished venueRequires access
AFLP AS A MODERN TECHNIQUE FOR DNA FINGERPRINTING AND IDENTIFICATION TULIPA CULTIVARS
I. O. Bondrea, Doru Pamfil, Sjaak van Heusden, J.M. van Tuyl, Fien Meijer‐Dekens, M. Bondrea, Radu E. SESTRAȘ, A. Raluca, Mariana Rusu, Bianca Patrascu, Valentin A. Bâlteanu
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Abstract
The recently introduced PCR- based DNA fingerprinting technique AFLP (Amplified Fragment Length Polymorphism ) allows the selective amplification of subsets of genomic restriction fr agments. AFLP has been used for multiple purposes such as construction of linkage maps. marker saturation at sp ecific genomic regions. analysis of genetic diversity and molecular phylogeny and cultivar identification. AF LP can be tailored by varying the number of selective nucleot ides added to core primers and can allow accurate amplification. even in complex template mixtures ge nerated from plant species with very large genomes. In this study Tulipa . a plant species with a very large genome (12 chro mosomes) was tested for adapting the AFLP protocol. The results indicated that obtain reprodu ctible AFLP patterns. clear and labor saving finger prints when seven selective nucleotides were used.