2000•Acta HaematologicaRequires access

Estimation of Stem Cell Fractions in Peripheral Blood Stem Cell Harvest by Using an SE-9000 Hematology Analyzer

Katsuyasu Saigo, T Sugimoto, Satoshi Takeuchi, Yoshiyuki Kosaka, Ryukichi Ryo, Shunichi Kumagai

Abstract

We inquired whether stem cell fractions in peripheral blood stem cell harvest could be precisely detected by using a hematopoietic progenitor cell (HPC) counting system applied to an automated hematology analyzer, SE-9000. Although there was an apparent increase in the HPCs 20 h after storage in nondiluted conditions, samples diluted with RPMI-1640 containing 1.0 mg/ml EDTA-2K showed a relatively stable number of HPCs. There was a significant relationship between HPCs and CD34 cells (n = 75, r = 0.769). This method may represent the least expensive and most time-effective way for stem cell estimation in harvest products.

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Key concepts: Hematology analyzer, Stem cell, Haematopoiesis, Hematology, Peripheral blood, CD34, Hematopoietic stem cell, Progenitor cell