1978•Nucleic Acids ResearchOpen access

Overmethylation of DNAs by the EcoRI methylase

Kathleen L. Berkner, William R. Folk

Abstract

EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI endonuclease activity, and thus is presumably at or near the EcoRI sequences (5') NAATTN.

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Key concepts: EcoRI, Biology, DNA, Restriction enzyme, Endonuclease, Genetics, Molecular biology