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Restriction enzyme analysis of bovine herpesvirus-1 DNA from an Indian isolate.

Praveen K. Gupta, A. Rai

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Abstract

An Indian isolate of BHV-1 was analyzed using several restriction endonucleases. The restriction pattern of BHV-1 DNA with HindIII, BamHI and EcoRI was found to be similar to other reported restriction endonuclease patterns for respiratory isolates of BHV-1. Thus, this isolate could be grouped under BHV-1.1 (respiratory form of BHV-1). Few restriction endonucleases yielded distinct restriction profile of BHV-1 DNA. The total molecular size of BHV-1 DNA for the isolate studied was also estimated to be within 136 kb to 138 kb.

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What this paper is about

An Indian isolate of BHV-1 was analyzed using several restriction endonucleases. The restriction pattern of BHV-1 DNA with HindIII, BamHI and EcoRI was found to be similar to other reported restriction endonuclease patterns for respiratory isolates of BHV-1. Thus, this isolate could be grouped under BHV-1.1 (respiratory form of BHV-1). Few restriction endonucleases yielded distinct restriction profile of BHV-1 DNA. The total molecular size of BHV-1 DNA for the isolate studied was also estimated to be within 136 kb to 138 kb.

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Available abstract

An Indian isolate of BHV-1 was analyzed using several restriction endonucleases. The restriction pattern of BHV-1 DNA with HindIII, BamHI and EcoRI was found to be similar to other reported restriction endonuclease patterns for respiratory isolates of BHV-1. Thus, this isolate could be grouped under BHV-1.1 (respiratory form of BHV-1). Few restriction endonucleases yielded distinct restriction profile of BHV-1 DNA. The total molecular size of BHV-1 DNA for the isolate studied was also estimated to be within 136 kb to 138 kb.

Key concepts: EcoRI, HindIII, Restriction enzyme, BamHI, Biology, Bovine herpesvirus 1, Restriction fragment, Restriction map

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