Technology and New Fluorescence Flow Cytometry Parameters in Hematological Analyzers

BACKGROUND: Modern hematological analyzers offer a series of new tests in addition to the differential blood cell count. Here, we provide a short overview of the technique and the new tests included in the hematological analyzers manufactured by the company Sysmex (Kobe, Japan). As introduced here, the resulting new extended blood cell count allows for the quantification of DNA- and RNA-rich, activated and/or precursor leukocyte subpopulations. METHODS: To compare the different methods of blood cell counting, repetitive cell counting is carried out by hematological experts using microscopy and using the XE-5000 hematological analyzer. RESULTS: The hematological analyzer delivers more-accurate results with respect to normal cells but microscopy is suited better to recognize and count pathological cells. CONCLUSION: With the new hematological analyzers, several new hematological tests are made available as screening tools that were not possible previously using standard cell-counting procedures.