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Studies on Purification of Total Flavonoids from the Marc of Blueberry by Macroporous Resins

Qian Hui-bi, Xiulan Xin, Lan Rong, Yadong Li

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Abstract

The absorption and desorption properties of six kinds of macroporous resin were studied.Of the six resins screened,HPD-600 was the best type to extract flavonoids from the marc of blueberry.The total content of flavonoids was measured by UV-spectrophotometric method.The result showed that the optimum condition to purify total flavonoids by HPD-600 was to control the flow velocity at 2 BV/h,pH value at 3.8 and with 60% ethanol as washing solution.The total flavonoids produced by this process was brown powder with total flavonoids purity up to 53.5%,which was 4.8 times higher than that of the original sample.

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What this paper is about

The absorption and desorption properties of six kinds of macroporous resin were studied.Of the six resins screened,HPD-600 was the best type to extract flavonoids from the marc of blueberry.The total content of flavonoids was measured by UV-spectrophotometric method.The result showed that the optimum condition to purify total flavonoids by HPD-600 was to control the flow velocity at 2 BV/h,pH value at 3.8 and with 60% ethanol as washing solution.The total flavonoids produced by this process was brown powder with total flavonoids purity up to 53.5%,which was 4.8 times higher than that of the original sample.

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Available abstract

The absorption and desorption properties of six kinds of macroporous resin were studied.Of the six resins screened,HPD-600 was the best type to extract flavonoids from the marc of blueberry.The total content of flavonoids was measured by UV-spectrophotometric method.The result showed that the optimum condition to purify total flavonoids by HPD-600 was to control the flow velocity at 2 BV/h,pH value at 3.8 and with 60% ethanol as washing solution.The total flavonoids produced by this process was brown powder with total flavonoids purity up to 53.5%,which was 4.8 times higher than that of the original sample.

Key concepts: Chemistry, Chromatography, Desorption, Ethanol, Absorption (acoustics), Flavonoid, Materials science, Organic chemistry

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