Study on flavonoids purification by macroporous resin and antioxidant activity of flavonoids from blueberry

To study the flavonoids purification by macroporous resin and antioxidant activity of flavonoids from blueberry.Four types of macroporous resin(AB-8、NKA-9、NKA-Ⅱ、D101)were used to purify flavonoids with static adsorption capacity and elution ratio as evaluation factors.Then the optimum purification conditions were investigated by L_9(3～4)orthogonal experimental design with recovery as evaluation factor.The antioxidant activity of the flavonoids from blueberry was tested by scavenging DPPH· and ·OH with VC and lutin as references.NKA-Ⅱ was the most suitable type of macroporous resin and the optimum conditions were as follows:the concentration of sample was 1.355mg·mL～(-1),the washing pH was 3,the flow rate of 70% ethanol was at 2mL·min～(-1),in this condition the purity of total flavonoids could reach 41.41%,33.94 times than before.The IC_(50) of antioxidant activity were 0.0217 and 0.1037mg·mL～(-1) respectively.Conclution was that the NKA-Ⅱ type macroporous resin showed better comprehensive absorption property,it can be used to purify the flavonoids from blueberry,the flavonoids from blueberry has strong antioxidant activity.