2009•Shengwu jiagong guochengRequires access

Microwave-assisted extraction of polysaccharides from fermented Cordyceps sinensis mycelium optimized by response surface analysis.

Xie Jianhua, Bin Shan, Zhang Wei-guo

Abstract

The microwave-assisted extraction process for polysaccharides extraction from fermented Cordyceps sinensis mycelium was optimized by using single factor experimental design.The effects of the ratio of water to material,the microwave power,and the extraction time were studied with the central composite experimental design.The predictive polynomial quadratic equation model was developed by SAS software.Optimal conditions were as follows: the ratio of solvent to material 12.2,microwave power 650.5 W,extraction time 11.8 min.Under the optimum condition,the yield of polysaccharides was up to 6.46%.The scavenging rate of the polysaccharides on 1,1-diphenyl-2-picrylhydrazyl reached 76% at the concentration of 1 mg/mL.

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Key concepts: Response surface methodology, Mycelium, Polysaccharide, Cordyceps, Extraction (chemistry), Fermentation, Central composite design, Yield (engineering)