2012•Journal of the South China Agricultural UniversityRequires access
Construction and expression of protokaryon expression plasmid for H and F genes of canine distemper virus isolated from mink.
Su Fengyan, Wen Tiefeng, Zong Ying, Quankai Wang
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Abstract
H and F genes were obtained by digesting the cloning plasmid pMD-18T-H and pMD-18T-F respectively.The recombinant protokaryon expression plasmids pET28a-H and pET28a-F were constructed by sub-cloning the objective gene into pET28a(+) vector.Recombinant protokaryon expression plasmids pET28a-H and pET28a-F were transformed into Escherichia coli Rosetta2(DE3) cell.Target genes were successfully expressed by SDS-PAGE detection.The size of the recombinant fusion proteins was 31 400(H) and 38 200(F) respectively.The recombinant fusion proteins showed positive reaction to anti-CDV canine serum by Western-blot detection.