Cloning and prokaryotic expression of F gene of canine distemper virus from ferret

The F gene was amplified by RT-PCR using RNA extracted from the canine distemper virus strain from ferrets, then cloned into pET-32a to construct an expression vector pET-32a-F. The constructed pET-32a-F was transformed into E.coli BL21 cells. An expected protein 55ku in size was expressed properly from pET-32a-F at 30℃ induced by 1.0mmol/L of IPTG, which was identified correctly by Western-blotting. The SDS-PAGE (analysis) showed that the product made up 35% of total bacterium proteins.