Construction of Prokaryotic Expression Vector of H Gene of Canine Distemper Virus

To offer accordance with H protein expression as diagnosis antigen,detect CDV specific antibody and provide the basis for CDV prevention,based on the constructed pMD18-H plasmid containing canine distemper virus N gene,the open reading frame of 1 824 bp was amplified by polymerase chain reaction(PCR) using one pair of primers.H gene and pET32a(+)were digested with the same procedure,then H gene was cloned into pET32a(+).The positive recombinant plasmid pET32a(+)-H was identified and the sequencing results showed that H gene was in the correct inserted position and open reading frame,it conformed that H gene was cloned into expression vector successfully.