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2009•Acta Horticulturae SinicaRequires access

Molecular Cloning,Characterization and Expression of A Manganese Superoxide Dismutase Gene From Ginkgo biloba

Hua Cheng, Linling Li, Xu Feng, Jie Chang, Yan Wang, Shuiyuan Cheng

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Abstract

The full-length cDNA sequence of Ginkgo biloba manganese superoxide dismutase gene(designated as GbMnSOD EF633506) were isolated from G.biloba for the first time. The full-length cDNA of GbMnSOD contained a 681 bp open reading frame(ORF) encoding a 226-amino-acid protein. The deduced protein have a predicted molecular weight of 25.5 kD and a calculated pI of 8.97. 3D structure modeling showed that GbMnSOD contain 12 helixes and 3 sheets and have a basketry motif in the enzyme core. Phylogenetic tree analysis revealed that GbMnSOD shared the same ancestor with other MnSOD. The result of southern analysis show that the MnSOD genes is encoded by a small gene family in the G. biloba. Northern hybridization analysis show that GbMnSOD expressed in leaves,stem,root and fruit and have the highest expression in leaves. The expression of MnSOD could be induced by ABA,IAA,sucrose,mannitol,NaCl and low temperature.

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What this paper is about

The full-length cDNA sequence of Ginkgo biloba manganese superoxide dismutase gene(designated as GbMnSOD EF633506) were isolated from G.biloba for the first time. The full-length cDNA of GbMnSOD contained a 681 bp open reading frame(ORF) encoding a 226-amino-acid protein. The deduced protein have a predicted molecular weight of 25.5 kD and a calculated pI of 8.97. 3D structure modeling showed that GbMnSOD contain 12 helixes and 3 sheets and have a basketry motif in the enzyme core. Phylogenetic tree analysis revealed that GbMnSOD shared the same ancestor with other MnSOD. The result of southern analysis show that the MnSOD genes is encoded by a small gene family in the G. biloba. Northern hybridization analysis show that GbMnSOD expressed in leaves,stem,root and fruit and have the highest expression in leaves. The expression of MnSOD could be induced by ABA,IAA,sucrose,mannitol,NaCl and low temperature.

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Available abstract

The full-length cDNA sequence of Ginkgo biloba manganese superoxide dismutase gene(designated as GbMnSOD EF633506) were isolated from G.biloba for the first time. The full-length cDNA of GbMnSOD contained a 681 bp open reading frame(ORF) encoding a 226-amino-acid protein. The deduced protein have a predicted molecular weight of 25.5 kD and a calculated pI of 8.97. 3D structure modeling showed that GbMnSOD contain 12 helixes and 3 sheets and have a basketry motif in the enzyme core. Phylogenetic tree analysis revealed that GbMnSOD shared the same ancestor with other MnSOD. The result of southern analysis show that the MnSOD genes is encoded by a small gene family in the G. biloba. Northern hybridization analysis show that GbMnSOD expressed in leaves,stem,root and fruit and have the highest expression in leaves. The expression of MnSOD could be induced by ABA,IAA,sucrose,mannitol,NaCl and low temperature.

Key concepts: Complementary DNA, Open reading frame, Ginkgo biloba, Gene, Molecular biology, Biology, Superoxide dismutase, Nucleic acid sequence

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