Molecular Cloning,Characterization and Expression of POD1 Gene from Ginkgo biloba L.

The full-length cDNA sequence of novel peroxidase1 gene(designed as GbPOD1,NCBI accession number:FJ599670) were isolated from Ginkgo biloba L.The coding region of the gene is 1 092 bp long,and its deduced mature protein consists of 329 amino acids with a predicted molecular mass of 35.8 kDa and a pI of 8.10.Phylogenetic tree analysis revealed that GbPOD1 shared the same ancestor with other PODs,could be classified as type III peroxidase.The result of southern analysis showed that GbPOD1 gene was encoded by a multi-gene family in Ginkgo biloba.The expression analysis by RT-PCR showed that GbPOD1 expressed in a tissue-specific manner in G.biloba.GbPOD1 was also found to be up-regulated by MeJA,CuSO4,CdCl2,MV,and mechanical wounding.These results indicate that the GbPOD1 has the potential to play a role in response to heavy metal and wounding against foreign attacks,was a multifunction gene.