2010•Xibei zhiwu xuebaoRequires access
Cloning and expression analysis of EPSP synthase gene from Ginkgo biloba L.
Hua Cheng, Linling Li, Yan Wang, Dezhi Jiang, Shuiyuan Cheng
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Abstract
The RACE technology was used for cloning the full-length cDNA of EPSP synthase gene from Ginkgo biloba.The cDNA sequence was 1 403 bp with a poly A tail,and contained a 1 035 bp open reading frame(ORF) encoding a 344 amino acid protein.Bioinformatics analysis predicted that it coded a protein of 36.87 kD,and its isoelectric point was 5.75.Phylogenetic tree analysis showed that the Ginkgo biloba EPSP synthase protein sequences was higly homology with the EPSP synthase of other species.RT-PCR analysis showed that GbEPSPS expressed in leaves,stems,roots and fruits,and had the highest expression in leaves and fruits,the next in stems.The least expression had been found in roots.The expression of GbEPSPS could be induced by glyphorose and UV-B.ABA could improve the expression of GbEPSPS first,but deduce later.The transcription levels were significantly induced by 42℃.