Determination of Puerarin and Paeoniflorin in Yanbao I Capsule by HPLC

[Objective] To establish the method for determining puerarin and paeoniflorin in Yanbao I capsule.[Method] The HPLC analysis used chromatography glass column Diamonsil C18(2)(4.6 mm×250 mm,5 μm) with the detection wavelength at 250 nm for puerarin and 230 nm for paeoniflorin and the column temperature was set at 25 ℃.Following the injection of sample solution,an elution was initiated at a flow rate of 1 ml/min with anhydrous methanol-water(V/V,28∶ 72) for Puerarin and acetonitrile-0.1% phosphoric acid(V/V,14∶ 86) as mobile phase.[Result] The linear range of puerarin was from 0.001 18 to 0.007 08 μg/ml(r=0.999 9).The average recovery of sample was 102.4% with RSD of 1.17%(n=6).The linear range of paeoniflorin was from 0.044 to 0.261 μg/ml(r=0.999 9).The average recovery of sample was 97.8% with RSD of 1.71%(n=6).In Yanbao I capsule,the content of puerarin and paeoniflorin could reach 0.055 and 0.168 mg/g,respectively.[Conclusion] The method is simple,accurate and repeatable,which is suitable for determining puerarin and paeoniflorin in Yanbao I capsule.