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Optimization of Extraction Methods for Genomic DNA in Eomecon chinantha Hance.

HU Xue-hua

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Abstract

[Objective]The research aimed to optimize the extraction methods for genomic DNA in Eomecon chinantha Hance.. [Method]The methods of CTAB and modified CTAB were chosen to extract the genomic DNA in Eomecon chinantha Hance. leaves.[Result]The results showed that the genomic DNA extracted by CTAB method contained many impurities, which were difficult to dissolve and easily degradable.The modified CTAB method was suitable to extract the genomic DNA in Eomecon chinantha Hance..The concentration and extraction rate of DNA from both fresh leaves and dry leaves preserved with silica gel at -80 ℃ were higher. [Conclusion]The study can provide the basis for studying the genetic diversity of Eomecon chinantha Hance..

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What this paper is about

[Objective]The research aimed to optimize the extraction methods for genomic DNA in Eomecon chinantha Hance.. [Method]The methods of CTAB and modified CTAB were chosen to extract the genomic DNA in Eomecon chinantha Hance. leaves.[Result]The results showed that the genomic DNA extracted by CTAB method contained many impurities, which were difficult to dissolve and easily degradable.The modified CTAB method was suitable to extract the genomic DNA in Eomecon chinantha Hance..The concentration and extraction rate of DNA from both fresh leaves and dry leaves preserved with silica gel at -80 ℃ were higher. [Conclusion]The study can provide the basis for studying the genetic diversity of Eomecon chinantha Hance..

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Available abstract

[Objective]The research aimed to optimize the extraction methods for genomic DNA in Eomecon chinantha Hance.. [Method]The methods of CTAB and modified CTAB were chosen to extract the genomic DNA in Eomecon chinantha Hance. leaves.[Result]The results showed that the genomic DNA extracted by CTAB method contained many impurities, which were difficult to dissolve and easily degradable.The modified CTAB method was suitable to extract the genomic DNA in Eomecon chinantha Hance..The concentration and extraction rate of DNA from both fresh leaves and dry leaves preserved with silica gel at -80 ℃ were higher. [Conclusion]The study can provide the basis for studying the genetic diversity of Eomecon chinantha Hance..

Key concepts: genomic DNA, DNA extraction, DNA, Extraction (chemistry), Chromatography, Chemistry, Biology, Biochemistry

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