2009•Chemia AnalitycznaRequires access

Determination of bovine serum albumin in the forum of its triple complex with Fe(bph)(phen)SO 4 -myristylpyridinium bromide by resonance light-scattering technique

Ling Li, Gongwu Song, Guangrong Fang

Abstract

A mixed complex Fe(bpy)(phen) 2+ was synthesized and resonance light-scattering (RLS) characteristics of a triple complex Fe(bpy)(phen)SO 4 -Myristylpyridinium bromide-bovine serum albumin were studied. When albumin was added, Fe(bpy)(phen) 2+ aggregated on the surface of albumin molecules at pH 5.5-7.0. As a result, RLS peaks at 400 nm and 470 nm were enhanced. The intensity of RLS signals was proportional to the concentration of bovine serum albumin. It was observed that the presence of myristylpyridinium sensitized the system. Determination limit was 0.02 mg L -1 .

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Key concepts: Chemistry, Bovine serum albumin, Bromide, Resonance (particle physics), Albumin, Detection limit, Serum albumin, Molecule