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Determination of Bovine Serum Albumin by a Resonance Light-Scattering Technique with a New Mixed-Complex La(C7H5O5S)(phen)3+

Ling Li, Gongwu Song, Guangrong Fang

Abstract

A new mixed-complex of La(C7H5O5S)(phen)3+ was synthesized. Resonance light-scattering characteristics of the interaction between La(C7H5O5S)(phen)3+ with bovine serum albumin (BSA) were studied. After BSA was added, aggregation of La(C7H5O5S)(phen)3+ on the molecular surface of the bovine serum albumin occurred at the pH 4.5∼6.2, which resulted in an enhanced resonance light-scattering (RLS) peak at 400 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of BSA.

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Key concepts: Bovine serum albumin, Resonance (particle physics), Light scattering, Chemistry, Scattering, Serum albumin, Analytical Chemistry (journal), Intensity (physics)