Response of Several Fungicides of Colletotrichum gloeosporioides Isolates Obtained from Persimmons in Sangju
Tae-Heon Lim, Yong-Hwa Choi
Abstract
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Tae-Heon Lim, Yong-Hwa Choi
Abstract
Open-access reader
상주 지역의 곶감용 감 생산 농가의 과수원으로부터 채집한 탄저병 감염 부위로부터 90 개의 감 탄저병균 (Colletotrichum gloeosporioides)을 분리하였다. 두 종의 benzimidazole계 약제 (thiophanate-methyl, carbendazim), 3 종의 egosterol 합성 저해제 (difenoconazole, myclobutanil, tebuconazole) 및 4 종의 보호 살균제 (propineb, mancozeb, chlorothalonil, dithianon) 등 총 9 종의 살균제에 대하여 분리한 균주들의 약제반응을 조사하였다. 상용농도로 약제가 첨가된 배지에서 대조구 대비 균사생육지수를 평가함으로서 조사하였다. Carbendazim( $415{\mu}g/ml$ )과 thiophanate-methyl( $750{\mu}g/ml$ ) 에 대한 반응은 각각 82%와 78%의 균주가 0.1 이하의 대조구 대비 균사생육지수를 보였다. 모든 실험 대상 균주는 myc1obutanil( $40{\mu}g/ml$ )과 tebuconazole ( $75{\mu}g/ml$ )이 첨가된 배지에서 균사생육이 완전히 억제되었으며, difenoconazole( $50{\mu}g/ml$ )이 첨가 배지에서는 단지 l 균주만이 0.03 의 대조구 대비 균사생장지수를 보였다. 4 종의 보호 살균제 중 propineb( $1,500{\mu}g/ml$ ) 에 대한 감수성이 가장 높게 나타났다. In 2005, 90 isolates of Colletotrichum gloeosporioides causing persimmons tree anthracnose were obtained from infected twigs and fruits of persimmon trees. Their responses to nine fungicides, consisting of two benzimidazoles (thiophanate-methyl, carbendazim), three ergosterol-biosynthesis inhibitors (difenoconazole, myclobutanil, tebuconazole), and four protective fungicides (propineb, mancozeb, chlothalonil, and dithianon), were investigated with relative mycelial growth index to untreated control on PDA treated with field application rate of each fungicide. At response to carbendazim ( $415{\mu}g/ml$ ) and thiophanate-methyl ( $750{\mu}g/ml$ ), 82% and 78% of isolates showed relative mycelial growth index under 0.1 to untreated control, respectively. All of them did not grow on PDA incorporated with myclobutanil ( $40{\mu}g/ml$ ) and tebuconazole ( $75{\mu}g/ml$ ). Only one isolate (PER 36) grew on PDA amended with difenoconazole ( $50{\mu}g/ml$ ), but its relative mycelial growth index to untreated control was very low with a values of 0.03. They were most sensitive to propineb ( $1,500{\mu}g/ml$ ) among four protective fungicides.
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상주 지역의 곶감용 감 생산 농가의 과수원으로부터 채집한 탄저병 감염 부위로부터 90 개의 감 탄저병균 (Colletotrichum gloeosporioides)을 분리하였다. 두 종의 benzimidazole계 약제 (thiophanate-methyl, carbendazim), 3 종의 egosterol 합성 저해제 (difenoconazole, myclobutanil, tebuconazole) 및 4 종의 보호 살균제 (propineb, mancozeb, chlorothalonil, dithianon) 등 총 9 종의 살균제에 대하여 분리한 균주들의 약제반응을 조사하였다. 상용농도로 약제가 첨가된 배지에서 대조구 대비 균사생육지수를 평가함으로서 조사하였다. Carbendazim( $415{\mu}g/ml$ )과 thiophanate-methyl( $750{\mu}g/ml$ ) 에 대한 반응은 각각 82%와 78%의 균주가 0.1 이하의 대조구 대비 균사생육지수를 보였다. 모든 실험 대상 균주는 myc1obutanil( $40{\mu}g/ml$ )과 tebuconazole ( $75{\mu}g/ml$ )이 첨가된 배지에서 균사생육이 완전히 억제되었으며, difenoconazole( $50{\mu}g/ml$ )이 첨가 배지에서는 단지 l 균주만이 0.03 의 대조구 대비 균사생장지수를 보였다. 4 종의 보호 살균제 중 propineb( $1,500{\mu}g/ml$ ) 에 대한 감수성이 가장 높게 나타났다. In 2005, 90 isolates of Colletotrichum gloeosporioides causing persimmons tree anthracnose were obtained from infected twigs and fruits of persimmon trees. Their responses to nine fungicides, consisting of two benzimidazoles (thiophanate-methyl, carbendazim), three ergosterol-biosynthesis inhibitors (difenoconazole, myclobutanil, tebuconazole), and four protective fungicides (propineb, mancozeb, chlothalonil, and dithianon), were investigated with relative mycelial growth index to untreated control on PDA treated with field application rate of each fungicide. At response to carbendazim ( $415{\mu}g/ml$ ) and thiophanate-methyl ( $750{\mu}g/ml$ ), 82% and 78% of isolates showed relative mycelial growth index under 0.1 to untreated control, respectively. All of them did not grow on PDA incorporated with myclobutanil ( $40{\mu}g/ml$ ) and tebuconazole ( $75{\mu}g/ml$ ). Only one isolate (PER 36) grew on PDA amended with difenoconazole ( $50{\mu}g/ml$ ), but its relative mycelial growth index to untreated control was very low with a values of 0.03. They were most sensitive to propineb ( $1,500{\mu}g/ml$ ) among four protective fungicides.
Key concepts: Myclobutanil, Tebuconazole, Fungicide, Carbendazim, Horticulture, Mancozeb, Chlorothalonil, Chemistry