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Genetic transformation of Bacillus strains close to bacillus subtilis and isolated from the soil

Coster Van, Yu.Yu. Kuzin, Yu. E. Kozlovskii, Alexander A. Prozorov

Abstract

Chromosomal and plasmid transformation was found in five out of 118 Bacillus strains, close or identical to Bacillus subtilis, and isolated from soil in Moscow or in the Moscow district. The efficiency of transformation in these strains was lower than that in derivatives of Bac. subtilis strain 168. In these strains the ability to undergo transformation was dependent on the rate of sporulation and the presence of restrictases. As in the case of Bac. subtilis 168 the strains isolated may be used as models in genetic transformation studies on Bac. subtilis.

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Key concepts: Bacillus subtilis, Transformation (genetics), Plasmid, Strain (injury), Microbiology, Bacillaceae, Bacillales, Biology