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[Restriction-modification systems in Bacillus strains related to Bacillus subtilis].

Iu E Kozlovskiĭ, A A Prozorov

Abstract

127 strains of bacilli sensitive to different phages of Bacillus subtilis were isolated from the soil of Moscow and its country-side. In 6 strains, restriction and modification systems were discovered which differed from these previously described for Bac. subtilis BsuR system. Two strains has identical restriction-modification systems, and one strain possessed two different systems. Using DNA from all 6 strains, it was possible to transform competent cells of Bac. subtilis RUB834. Two of these 6 strains could serve as recipients in transformation and transfection experiments.

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Key concepts: Bacillus subtilis, Bacilli, Transformation (genetics), Strain (injury), Microbiology, Bacillaceae, Biology, DNA