Effects of Yeast Fermentation on the Production of Soy-oilgosaccharides from Bean Cooking Water

Bean cooking water was used as a raw material for the production of soy-oligosaccharides. To maximize the yield of the physiologically functional oligosaccharides such as raffinose and stachyose, a fermentation process was introduced to reduce sucrose content. Yeast strains utilizing sucrose, but scarcely affecting the raffinose and stachyose for the growth were initially selected to reduce the sucrose content in the bean cooking water. The selected strains were Saccharomyces cerevisiae ATCC 9763, S. cerevisiae KCTC 7039 and Hansenula anomala KFRI 626. Bean cooking water with 2% inoculation volume of these yeasts was fermented until 48 hrs, respectively. The results of sugar analysis according to the fermentation time showed, bean cooking water treated with S. cerevisiae KCTC 7039 and H. anomala KFRI 626 used almost sucrose without decreasing stachyose for the growth.