PREPARATION OF FUNCTIONAL SOYBEAN OLIGOSACCHARIDE FROM SOYBEAN MOLASSES BY FERMENTATION

The preparation conditions of functional soybean oligosaccharide from soybean molasses were studied by fermentation using S.cerevisiae to remove sucrose in the soybean molasses.A preferred S.cerevisiae was selected.The optimum fermentation conditions of soybean molasses using the preferred S.cerevisiae were determined by orthogonal experiment as follows: fermentation temperature 30 ℃,fermentation time 48 h,molasses concentration 10%,molasses pH 5.0,and inoculation amount 5%.Under the optimum conditions,the sucrose removal rate was reach to about 90%;the raffinose retention rate and the stachyose retention rate were respectively 85.70% and 94.30%;and the relative content of raffinose and stachyose was 84.24%,which was higher than that before the fermentation by 52.5%.The ethanol content in the fermentation broth was 7.68 g/L,and the ethanol yield was 55.7%.