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Measurement and Control of Free Calcium Inside Small Intact Cells

Roger Y. Tsien

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Abstract

The use of dye to measure intracellular free calcium concentration was discussed. Difficulty in measuring the calcium is caused by two problems: the small amount of calcium available for testing, and the selectivity to bind calcium at 100 nanomolar excludes substances from entering the cell.

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What this paper is about

The use of dye to measure intracellular free calcium concentration was discussed. Difficulty in measuring the calcium is caused by two problems: the small amount of calcium available for testing, and the selectivity to bind calcium at 100 nanomolar excludes substances from entering the cell.

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Available abstract

The use of dye to measure intracellular free calcium concentration was discussed. Difficulty in measuring the calcium is caused by two problems: the small amount of calcium available for testing, and the selectivity to bind calcium at 100 nanomolar excludes substances from entering the cell.

Key concepts: Calcium, Chemistry, Biophysics, Calcium in biology, Biochemistry, Biology, Organic chemistry

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