1999•Current Protocols in ToxicologyRequires access

Measurements of Intracellular Free Calcium Concentration in Biological Systems

Karlene K. Gunter, Thomas E. Gunter

Abstract

Intracellular calcium plays a role in signaling and as a second messenger in many types of cells and its concentration is closely regulated in cells. Two techniques for measuring intracellular free calcium are using fluorescent ratiometric and nonratiometric probes are described in this unit: fluorescent spectroscopy to measure calcium concentrations in a suspension of cells and fluorescent digital imaging microscopy (FDIM) to measure calcium concentrations in plated cells.

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Key concepts: Intracellular, Calcium, Fluorescence, Calcium in biology, Biophysics, Fluorescence microscope, Chemistry, Second messenger system