1995•Unpublished venueRequires access

Effect of Some Factors Affecting Callus Formation of Red Clover

Dae Young Son, Jin Ki Jo

Abstract

This experiment was conducted to determine factors affecting callus formation of some varieties of red clover. Growth regulators. basal medium, medium pH, agar concentration, and plant age for explant were investigated. The result obtained were as follows. As auxin source, 0.1 mg/ l picloram was found to be effective on callus formation. Callus formation was the highest on PC medium among several growth media. The callus produced on PC medium was more friable than those produced on the other media. The medium pH 5.8 gave the best response for callus formation. The effective seedling age for callus formatiom was around 5-7 days old. In agar concentration, 0.5-0.8%(WN) was suitable for callus formation.

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Key concepts: Callus, Picloram, Agar, Explant culture, Botany, Seedling, Chemistry, Auxin