[Establishment of immortal lymphoblastoid cell bank of keloids pedigree].
Song Mei, Jianhua Gao, Xin Yan, Xiaojun Liu, Yang Chen
Abstract
Song Mei, Jianhua Gao, Xin Yan, Xiaojun Liu, Yang Chen
Abstract
OBJECTIVE: To provide perpetual research materials for long term studies by establishing immortal lymphoblastoid cell bank of keloids pedigree. METHODS: The immortal lymphoblastoid cell lines of keloids pedigree were established by Epstein-Barr virus transformation of peripheral blood B lymphocytes. RESULTS: 27 immortal lymphoblastoid cell lines of keloids pedigree were obtained successfully, all of the immortal lymphoblastoid cell lines were successfully revivificated after been frozen in liquid nitrogen. CONCLUSIONS: It is important to establish immortal lymphoblastoid cell bank of keloids pedigree and provide long-term DNA materials for deep study of keloids in the future.
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OBJECTIVE: To provide perpetual research materials for long term studies by establishing immortal lymphoblastoid cell bank of keloids pedigree. METHODS: The immortal lymphoblastoid cell lines of keloids pedigree were established by Epstein-Barr virus transformation of peripheral blood B lymphocytes. RESULTS: 27 immortal lymphoblastoid cell lines of keloids pedigree were obtained successfully, all of the immortal lymphoblastoid cell lines were successfully revivificated after been frozen in liquid nitrogen. CONCLUSIONS: It is important to establish immortal lymphoblastoid cell bank of keloids pedigree and provide long-term DNA materials for deep study of keloids in the future.
Key concepts: Lymphoblast, Cell culture, Cell, Virus, Medicine, Biology, Immunology, Genetics