Establishment and preservation of immortal lymphoblastoid cell lines of the primary open angle glaucoma pedigree in Chongqing

Objective To further explore the pathologic mechanisms of primary open angle glaucoma(POAG)and establish and preserve the immortal lymphoblastoid cell lines in a large POAG pedigree of Chongqing.Methods The immortal lymphoblastoid cell lines were established by Epstein-barr virus(EBV) transformation of peripheral blood lymphocytes and addition of cyclosporine A.In this present study,39 immortal cell lines of the large POAG pedigree were established,including 11 POAG patients and 28 unaffected relatives (21 males and 18 females).Results The immortal lymphoblastoid cell lines has been proved to be successful in above 92.3% of blood samples and in almost 100% for rejuvenate cells.Conclusion The successfully established immortal lymphoblastoid cell lines are important for cytogenetics and molecular biology study of POAG.