The effect of bcl-2 gene and GDNF of rats after focal cerebral ischemia reperfusion injury.

Objective To observe the effect of bcl-2 gene and glial cell line-derived neurotrophic growth factor (GDNF)in rats with focal cerebral ischemia reperfusion injury. Methods Middle Cerebral Artery Occlusion(MCAO)models were made by reforming Longa method in 40 healthy Wistar rats,Reperfusion was implemented two hours later.The rats were randomly divided into 4 groups:control group、bcl-2 group、GDNF group and GDNF+bcl-2 group.Three hours after reperfusion,10 μg PlXSN-bcl-2 gene was injected into internal carotid artery, 10 μl GDNF was injected into the lateral cerebral ventricle under stereotaxis,3 or 14 days later,5 rats in each group were sacrificed respectively,GDNF and bcl-2 protein expression were determined through immunohistochemistry,and the distribution of apoptosis neurons was checked out by TUNEL techniques. Results There was significant increase in the expression of bcl-2 protein and the expression of GDNF not only in bcl-2+ GDNF groups compared to other groups(P 0.05),but also in bcl-2 group and GDNF group compared to control group (P 0.05);There was significant decrease in the apotosis of nerve cells not only in bcl-2+ GDNF group compared to other groups (P 0.05),but also in bcl-2 group and GDNF group compared to control group(P 0.05). Conclusion bcl-2 gene and GDNF maybe protect focal ischemic brain tissue and accelerate repair of central nervous system damage through reduction of neuronal apoptosis of nerve cells in the brain so as to avoid cerebral ischemia reperfusion injury of brain cells.