ScholarLens Back to search
2004•PubMedRequires access

Evaluation of cisplatin-DNA crosslinks formation with UV-C application by the alkaline Comet-assay.

Galina Hovhannisyan, Tigran S Haroutunyan, Rouben Arutyunyan

Open publisher page 12 citations

Abstract

UNLABELLED: The AIM of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors. METHODS: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The RESULTS obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration. CONCLUSION: The Comet-assay with UV-C application is a useful tool to detect cis-DDP- induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.

About this research paper

What this paper is about

UNLABELLED: The AIM of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors. METHODS: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The RESULTS obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration. CONCLUSION: The Comet-assay with UV-C application is a useful tool to detect cis-DDP- induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.

Why it matters

OpenAlex reports 12 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

UNLABELLED: The AIM of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors. METHODS: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The RESULTS obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration. CONCLUSION: The Comet-assay with UV-C application is a useful tool to detect cis-DDP- induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.

Key concepts: Comet assay, Cisplatin, DNA damage, DNA, Chemistry, In vivo, Gel electrophoresis, Molecular biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Evaluation of cisplatin-DNA crosslinks formation with UV-C application by the alkaline Comet-assay. — Research Paper | ScholarLens