Study on callus induction and plant regeneration by shoot tip of Cunninghamia lanceolata.

The shoot tips sprouted from the trunk base of the superior individual plants of Cunninghamia lanceolata were applied as the explants to induce the callus on the improved medium MS+KT 0.2 mg/L+2,4-D 1.0 mg/L.The results showed that the induction rate reached 86.7%;the culture medium for differentiation and multiplication of adventitious bud was the improved MS+KT 1.0 mg/L+IBA 0.5mg/L,whose differentiation coefficient reached 3.42.The culture medium for rooting to be applied 30 days later was the improved 1/2MS+IBA 0.2 mg/L+ NAA 0.5 mg/L,by which the rooting rate was 93% within 25 days.The rooted seedlings were transplanted to the seedbed covered by 3 cm yellow soil for further cultivation,and the transplanting survival rate was over 90%.