2008•The Sydney eScholarship Repository (The University of Sydney)Open access
Stem rust resistance in South African wheat cultivars
Pretorius Za, Jin Ye, R. Prins, Bender Cm, L. Herselman
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Abstract
The appearance and anticipated spread of race TTKS (syn.Ug99) of Puccinia graminis f. sp.tritici have renewed interest in breeding for durable resistance to stem rust of wheat.In an attempt to determine the current status of stem rust resistance in South African (SA) bread wheat, 67 cultivars and lines were tested with US and East African races of P. graminis f. sp.tritici.Entries were also screened with DNA markers associated with Sr24 (Sr24#50) and Sr31 (iag95).Sr2 DNA marker (stm559n) data were compared with seedling chlorosis scores to validate the use of this marker in SA genotypes.Most cultivars interacted differentially with the races tested.DNA marker analysis confirmed the presence of Sr31 in seven and Sr24 in 12 entries.Stm559n for Sr2 reliably amplified the correct allele in most local and control lines.However, in several instances the Sr2-associated allele was amplified in presumably non-Sr2 carrying cultivars.This study emphasized that diversification of resistance sources is needed as few SA wheat entries appear to have a broadbased resistance to stem rust.
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