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2010•Unpublished venueRequires access

Construction of insect-resistant gene plant expression vector

Zhi-Nian Deng, Wei YuanWen, Chengmei Huang, Pan YouQiang, Wei-Li Lü, Yang‐Rui Li

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Abstract

Based on plasmids of pCAMBIA1200,pBI121,pMMAR and pC1300MAR,plant intermediate vector pC2MARHyg was constructed by digestion and ligation.Plant expression vector pC2MAR-AVAc containing Ubi-1 promoter,direct duplication MAR sequence and insect-resistant gene(AVAc),were constructed using pAVAc plasmid,pC2MARHyg vector and pGA1611 vector.The constructed vector was transformed to Agrobacterium EHA105.The newly constructed plant expression vector can be used in molecular breeding programs tailored for developing insect-resistance crop plants.

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What this paper is about

Based on plasmids of pCAMBIA1200,pBI121,pMMAR and pC1300MAR,plant intermediate vector pC2MARHyg was constructed by digestion and ligation.Plant expression vector pC2MAR-AVAc containing Ubi-1 promoter,direct duplication MAR sequence and insect-resistant gene(AVAc),were constructed using pAVAc plasmid,pC2MARHyg vector and pGA1611 vector.The constructed vector was transformed to Agrobacterium EHA105.The newly constructed plant expression vector can be used in molecular breeding programs tailored for developing insect-resistance crop plants.

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Available abstract

Based on plasmids of pCAMBIA1200,pBI121,pMMAR and pC1300MAR,plant intermediate vector pC2MARHyg was constructed by digestion and ligation.Plant expression vector pC2MAR-AVAc containing Ubi-1 promoter,direct duplication MAR sequence and insect-resistant gene(AVAc),were constructed using pAVAc plasmid,pC2MARHyg vector and pGA1611 vector.The constructed vector was transformed to Agrobacterium EHA105.The newly constructed plant expression vector can be used in molecular breeding programs tailored for developing insect-resistance crop plants.

Key concepts: Vector (molecular biology), Plasmid, Expression vector, Biology, Gene, Agrobacterium, Transformation (genetics), Genetics

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