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Influencing Factors of Callus Induction on Blueberry Anther in Vitro

You LaiQiu, Xiaoyan Li, Zhidong Zhang, Yadong Li, Wu Lin, Liu Haiguang

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Abstract

Effects of hormone,florescence,corolla state,light and pH value on inducing callus of Patriot blueberry anther in vitro were studied.The results showed that:WPM+BA 0.5 mg/L+2,4-D 1.0 mg/L medium was most suitable for anther callus induction.Corolla with 2:1 of length and width during full flowering stage was the best period for sample collection.Culture under darkness condition promoted the callus induction of blueberry anther,with an induction rate of 56.67%.There were no obvious differences in callus induction rate when medium pH value varied from 4.98 to 5.50.

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What this paper is about

Effects of hormone,florescence,corolla state,light and pH value on inducing callus of Patriot blueberry anther in vitro were studied.The results showed that:WPM+BA 0.5 mg/L+2,4-D 1.0 mg/L medium was most suitable for anther callus induction.Corolla with 2:1 of length and width during full flowering stage was the best period for sample collection.Culture under darkness condition promoted the callus induction of blueberry anther,with an induction rate of 56.67%.There were no obvious differences in callus induction rate when medium pH value varied from 4.98 to 5.50.

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Available abstract

Effects of hormone,florescence,corolla state,light and pH value on inducing callus of Patriot blueberry anther in vitro were studied.The results showed that:WPM+BA 0.5 mg/L+2,4-D 1.0 mg/L medium was most suitable for anther callus induction.Corolla with 2:1 of length and width during full flowering stage was the best period for sample collection.Culture under darkness condition promoted the callus induction of blueberry anther,with an induction rate of 56.67%.There were no obvious differences in callus induction rate when medium pH value varied from 4.98 to 5.50.

Key concepts: Callus, Stamen, Darkness, In vitro, Botany, Horticulture, Chemistry, Biology

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