2007BioTechniquesOpen access

Sequencing of avian influenza virus genomes following random amplification

Claudio L. Afonso

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Abstract

Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols.

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What this paper is about

Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols.

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Available abstract

Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols.

Key concepts: Biology, Virus, Genome, Virology, Influenza A virus subtype H5N1, DNA sequencing, Influenza A virus, Avian influenza virus

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